Check out Ellen O’Shaughnessy’s first author work now on BioRxiv, in collaboration with David Adalasteinsson, Christophe Leterrier, and Brad Zuchero. We describe a new toolset for robust and versatile analysis of exocytosis mediated by different vSNAREs in primary neurons, primary oligodendrocytes, and migrating melanoma cells.
Check out Laura McCormick’s first author work now on BioRxiv, in collaboration with Nick Brown and Dave Kovar. We identified 2 ubiquitination sites on VASP (K240 and K286) and created purified, ubiquitinated VASP mimics. Using several in vitro biochemical assays, we showed multi-monoubiquitination (mmUb) reduces the ability of VASP to bind, bundle, and elongate actin filaments. We also electroporated our purified protein into MEFs. Compared to unmodified protein, the presence of multi-monoubiquitinated VASP altered cell spreading morphology and showed diminished localization to lamellipodia/filopodia. Collectively, our results show how multi-monoubiquitination negatively regulates VASP activity and influences actin dynamics in cells.
Stephanie was an invited speaker at the Neurotrophic Mechanisms in Health and Disease GRC, where she presented Laura McCormick’s beautiful thesis work.
- Stephanie visits University of California Los Angela, invited by Dr. Samantha Butler to give a seminar in the Integrative Center for Neural Repair Seminar Series
We just received a Notice of Award for a new R21 to study netrin-1 chemotaxis and haptotaxis! Woohoo
Two new BBSP students are joining the lab and the Cell Biology and Physiology Curriculum. Welcome to Anca Frasineanu and Sam Ryken!
Senior Undergraduate, Elliot Evans defended his honors thesis in Neuroscience. Congratulations Elliot
The long awaited workshop funded by EMBO/IUBMB/IBRO/ISN on the Emerging Concepts of the Neuronal Cytoskeleton, that Stephanie has been organizing the past three years with Christophe Letterier, Carlos Wilson, and Christian Gonzalez Billault is finally here! Laura McCormick will be presenting a short talk and two posters, and Chris Ho is presenting a poster.
Chris Ho received a CFAC award for $10,000 to fund an exciting project using light sheet microscopy to examine axon branching in the mouse cortex in vivo. Congratulations Chris!
Stephanie, Laura, Sampada, Chris, and Kimberly all attended the CellBio22 Meeting by ASCB/EMBO, with posters, sessions, and so much great science. It was great to be in person again!