Confocal Sections

WT adult born neuron in DG

Axon Branching in the Corpus Callosum increases in absence of TRIM9 Download Full Resolution TIFS

DCC clustering

Netrin dependent clustering fails to occur in absence of TRIM9

Netrin Dependent Clustering off non-ubiquitinatable DCC-KR

Exocytosis

TRIM9 deletion increase VAMP2-mediated exocytosis

Automated VAMP2-pHluroin exocytic dection (red) and rejected events (blue)

VAMP2-pHluorin mediated exocytosis over developmental time

Axon Turning

Netrin-dependent axon turning

A gradient of DUB inhibitor induces repulsive axon turning

Colocalization
Colocalization of GFP-VASP and TRIM9deltaRING

FRAP of GFP-VASP at growth cone filopodia tip